All trace elements critical to continued functioning of photoautotrophs, including nickel, are provided in Isol8 MT, which offers a simpler method of dosing the aforementioned elements in ratios mirroring those of natural seawater, and is recommended for general husbandry of recirculating marine ecosystems. Isol8 Ni is recommended for adjustment of nickel values in accordance with accurate ICP analysis.
The rate of ion depletion due to photoautotroph uptake is driven by various chemical and physical factors which are unique to each independently-operating recirculating system, and which are constantly changing as photoautotroph biomass increases and decreases. In recirculating systems dosed with ammonium or urea as a nitrogen source, the rate of nickel depletion through biochemical processes may exceed the rate at which the element is dosed via Isol8 MT. In such instances, verify the nickel value in the system (refer to our recommendation for ICP analysis, above) and dose Isol8 Ni according to Reference Standards shown below.
Monitoring (daily) the system's concentrations of elements provided by this formulation, as well as the concentration of nitrate, and referencing apparent changes in coloration of zooxanthellate cnidarians will enable User to adjust dosing of this formula to suit system requirements, improving overall effectiveness and resulting in less wasted solution.
Users unable to perform analyses for elements provided by this formula are recommended to monitor nitrate concentration and apparent changes in coloration of zooxanthellate cnidarians, adjusting dosage to suit system requirements. This visual approach to dosing is fastest, and for many systems will be adequate.
Recommended is diluting an appropriate volume of this solution into a larger volume of purified water (with a purity of at least 98%), and applying this diluted solution to the system with an automated dosing system throughout the photoperiod. Dosing frequency is directly proportional to stability of ionic concentrations and, resultantly, gradual changes in the coloration (manifesting as intensification) of coloration of zooxanthellate cnidarians. A 1 - 10 s drip interval during the photoperiod provides adequate ionic stability, and is recommended.
If no automated dosing system is available, then apply solution manually each day. Dosing frequency is directly proportional to stability of ionic concentrations and, resultantly, consistent changes in coloration of zooxanthellate cnidarians. Dosage volume per 24 h is divided by the dosing frequency.
It is not recommended that this formulation be dosed fewer than four times weekly.
Reference Standards: 1 drop (0.05 mL) Isol8 Ni diluted in 26.4 gal. (100 L) water yields 0.001 ppm increase of [Ni]. Undiluted, 1 drop treats a volume of 28.1 gallons (106.5 L) at the reference standard of 4.70x10-4 ppm.